Whole genome analysis and in vivo safety assessment of probiotic candidate Lactobacillus acidophilus L177

Authors: Yunjiang Liu1 et al.

Journal:BMC Microbiol 2025

Research Areas: Microbiology Discipline

Cell Lines: Lactic acid bacteria (LAB)

Summary: Probiotics, particularly strains of lactic acid bacteria (LAB), are recognized for their beneficial effects on gut health. Lactobacillus acidophilus L177, a promising probiotic strain isolated from canine feces, was evaluated for safety and probiotic properties through whole genome sequencing and a 28-day subacute oral toxicity study in C57BL/6 mice. In this study, the Halo Counter HD-4 Cell Counter (Hiscore Inc, China) was used for counting and quality control to ensure the validity of whole-genome sequencing of lactic acid bacteria.In conclusion, L. acidophilus L177 demonstrated excellent safety profiles and probiotic potential based on in vivo toxicity studies and whole-genome analysis. Future studies should explore the strain’s efficacy in clinical settings, focusing on its probiotic effects, such as gut health modulation and immune support. Additionally, long-term safety assessments and mechanistic studies on probiotic action will further validate the utility of L. acidophilus L177 as a safe and effective probiotic.

Keywords: Lactobacillus acidophilus, Subacute oral toxicity study, Whole genome sequencing, Probiotics, Lactic acid bacteria